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. 2020 Apr 6;19(5):2071–2079. doi: 10.1021/acs.jproteome.0c00003

Figure 2.

Figure 2

Optimization of input bead amount and probing of the enrichment method based on Halo-tagged recombinant Cas9 protein. Number of unique cross-links within Cas9 (A) or unique monolinked peptides (as given by MeroX) and linear peptides (as given by MS Amanda) (B) after linking 20 μg of the recombinant protein with 0.5 mM DSBSO with or without enrichment (control) using the indicated bead slurry volumes. Number of unique cross-links on Cas9 (C), or unique detected monolinked and linear peptides (D) after linking 20 μg Cas9 each and spiking into tryptic HEK peptides in excess as indicated, enriched using 12 μL of DBCO bead slurry each. Results are filtered for 5% FDR, n = 1.