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. 2020 Apr 6;19(5):2071–2079. doi: 10.1021/acs.jproteome.0c00003

Figure 3.

Figure 3

Probing enrichment on a more complex system using cross-linked E. coli ribosome. Number of unique cross-links detected within ribosome-shotgun database (A), number of detected unique linear peptides without a DSBSO modification from MS Amanda and number of unique monolinked peptides from MeroX (B), ratio of detected cross-linked (XlinkX) over linear peptides (MS Amanda) (C) without enrichment (control), after DBCO bead enrichment (enriched) or in the remaining supernatant over the beads after click reaction (depleted). Twenty μg E. coli ribosome each were linked using 1 mM DSBSO. Bars indicate the average values, with standard deviation depicted as error bars, 5% FDR, n ≥ 3.