TABLE 1.
Distribution of cell wall epitopes for antibodies specific for various polymers in P. nudum stem tissues.
| Antibody/references | Antigen/epitope | Tissue of P. nudum stem | ||||||||
| Epidermis | Outer cortex | Middle cortex | Inner cortex | Endodermis | Phloem | Xylem | ||||
| ML + PCW | SCW | ML + PCW | SCW | |||||||
| LM21 (Marcus et al., 2010) | (galacto) (gluco)mannan | +++ | +++ | +++ | +++ | +++ | ±1 | +1 | ± | ± |
| LM11 (McCartney et al., 2005) | (1→4)-β-D-xylan/arabinoxylan | +2 | − | − | +++ | − | − | − | − | +++ |
| LM15 (Marcus et al., 2008) | XXXG motif of xyloglucan | +++ | +++ | +++ | ++3 | + | + | +++ | ± | ± |
| JIM5 (Knox et al., 1990) | Partially Me-HG/de-esterified HG | ++ | ++ | − | ± | − | − | − | − | − |
| JIM7 (Knox et al., 1990) | Partially Me-HG | +++ | +++ | ++ | + | + | + | +++ | + | − |
| LM5 (Jones et al., 1997) | (1→4)-β-D-galactan | ++ | ++ | − | +++3 | + | − | − | − | +++ |
| LM6 (Willats et al., 1998) | (1→5)-α-L-arabinan | +2 | − | − | ++3 | ± | − | − | − | − |
| JIM14* (Knox et al., 1991) | Arabinogalactan protein | − | − | − | − | − | − | − | − | − |
| BG1* (Meikle et al., 1994) | (1→3)-(1→4)-β-D-glucan | − | − | − | − | − | − | − | − | − |
+++ very strong labeling; ++ strong labeling; + weak labeling; ± very weak and scattered labeling; – no labeling; 1restricted to primary cell wall and cell corners; 2restricted to stomata cell; 3restricted to the innermost cell wall region. ∗JIM14 and BG1 did not bind to any tissue in P. nudum stem but worked well on the positive controls.