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. 2020 Apr 13;14(4):4444–4455. doi: 10.1021/acsnano.9b10033

Figure 2.

Figure 2

Exogenously added EVs localize in membrane-bound compartments in HEK293T acceptor cells. (A) HEK293T cells incubated for 12 h with GFP-CD63 EVs show a punctate staining pattern in the cytosol (scale bars, 10 μm). (B) Correlative light (green) and EM (greyscale) microscopy for ultrastructural analysis of the internalized EVs (GFP punctae) of the boxed area in (A) (scale bars, 5 μm). (C) Underlying ultrastructures of areas 1–3 in (B) reveal vesicular structures. Additional snap shots available in Supplementary Figure 3B. (Scale bars, 0.2 μm.) (D) Structures given in (C) are labeled with QDs (indicated by arrowhead) following anti-GFP immunolabeling, confirming the presence of GFP-CD63 EVs within the vesicular structures (scale bars, 0.2 μm). All EM data sets at full resolution are available viawww.nanotomy.org.