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. 2020 Apr 28;10:552. doi: 10.3389/fonc.2020.00552

Figure 2.

Figure 2

MSCs induce activation (phosphorylation) of PDGFR-α with downstream activation of AKT in OSCC. (A) Downstream activation of p-AKT levels were observed at serine 473 (S473) in co-cultures from JHU-012, and −019 compared to cancer cells grown alone. (n = 3; p < 0.025). (B) A more robust increase in p-AKT levels were also observed at threonine 308 (T308), corresponding to the catalytic site for AKT in JHU-012 and −019 grown in co-culture with MSCs (n = 3; *p < 0.02; **p < 0.007). (C) Inhibition of PDGFR-α results in decreased expression of activated AKT. JHU-012 were grown alone or in 1:1 co-culture with MSCs for 6 days in the presence of PDGFR- α neutralizing antibodies or isotype control and expression of p-PDGFR-α (Y754), pAKT (S473) and p-AKT (T308) measured by Western immunoblotting. There was a significant decrease in p-PDGFR-α, p-AKT (S473), and pAKT (T308) expression following inhibition of PDGFR-α neutralizing antibodies compared to isotype control (n = 3; p < 0.03).