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. 2018 May 2;315(2):R323–R335. doi: 10.1152/ajpregu.00444.2017

Fig. 3.

Fig. 3.

Expression of lysosome proteins is not impaired in Zn transporter 2 (ZnT2)-null (ZnT2ko) mice. A: relative gene expression of Ctsd (cathepsin D), Lamp1 [lysosome-associated membrane protein 1 (Lamp1)], Mcoln1 [mucolipin 1 (TRPML1)], Ppt1 (palmitoyl-protein thioesterase 1), and Tnfa (TNFα) in involuting mammary glands from wild-type (WT) and ZnT2ko mice. Data were normalized to β-actin.  Values are means (SD); n = 5 mice/genotype. B: representative immunoblot of Lamp1 in total membrane fractions of mammary glands prepared from WT and ZnT2ko involuting mammary glands. Data were normalized to β-actin. Values are means (SD); n = 4 mice/genotype. Experiment was repeated 3 times. β-Actin served as a loading control. C: representative immunoblot of pro- and mature cathepsin B (catB) expression in cell lysates prepared from WT and ZnT2ko involuting mammary glands. Data represent sum of pro- and mature catB abundance relative to β-actin. Values are means (SD); n = 4 mice/genotype. Experiment was repeated 3 times.