Fig. 6.

Impaired lysosome acidification with loss of Zn transporter 2 (ZnT2) is not a result of Zn toxicity. A: representative images of LysoSensor yellow/blue DND-160 fluorescence in untransfected (Mock; i, ii, vi, and vii) and ZnT2-attenuated (ZnT2KD; iii–v and viii–x) mammary epithelial cells (MECs) treated with TNFα (+TNFα) or TNFα + N,N,N′,N′-tetrakis(2-pyridylmethyl) ethylenediamine (+TNFα +10 µM TPEN) or unstimulated (untreated) MECs. Magnification ×63; scale bars = 25 µm (i–v) and 8 µm (vi–x). B: vesicular pH. Values are means ±  SE of all vesicles from five, ×63 images/group; n = 5 replicates per group. *P < 0.05; ****P < 0.0001.