Table 1.

Primer sequences for CLEAR network genes assessed using RT-PCR

	Primer Sequence
Ctsd
Forward	5′-TACTCAAGGTATCGCAGGGTG-3′
Reverse	5′-CCAATGAAGACATCGCCCAG-3′
Lamp1
Forward	5′-GTAACAACGGAACCTGCCTG-3′
Reverse	5′-TCTGGTCACCGTCTTGTTGT-3′
Mcoln1
Forward	5′-GGCATTGAGGCAAAGAACCT-3′
Reverse	5′-GAATGACACCGACCCAGACT-3′
Ppt1
Forward	5′-CTCTGCTGCCTTGGTGCT-3′
Reverse	5′-AGCTGTCCCCCATCCCAT-3′
Tnfa
Forward	5′-AGGGTCTGGGCCATAGAACT-3′
Reverse	5′-CCACCACGCTCTTCTGTCTAC-3′
Actb
Forward	5′-CTCTCAGCTGTGGTGGTGAA-3′
Reverse	5′-AGCCATGTACGTAGCCATCC-3′

CLEAR, coordinated lysosome expression and regulation; Ctsd, cathepsin D; Lamp1, lysosome-associated membrane protein; Mcoln1, mucolipin 1 (TRPML1); Ppt1, palmitoyl-protein thioesterase 1; Tnfa, TNF-α; Actb, β-actin.