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. 2018 May 2;315(2):R323–R335. doi: 10.1152/ajpregu.00444.2017

Table 1.

Primer sequences for CLEAR network genes assessed using RT-PCR

Primer Sequence
Ctsd
Forward 5′-TACTCAAGGTATCGCAGGGTG-3′
Reverse 5′-CCAATGAAGACATCGCCCAG-3′
Lamp1
Forward 5′-GTAACAACGGAACCTGCCTG-3′
Reverse 5′-TCTGGTCACCGTCTTGTTGT-3′
Mcoln1
Forward 5′-GGCATTGAGGCAAAGAACCT-3′
Reverse 5′-GAATGACACCGACCCAGACT-3′
Ppt1
Forward 5′-CTCTGCTGCCTTGGTGCT-3′
Reverse 5′-AGCTGTCCCCCATCCCAT-3′
Tnfa
Forward 5′-AGGGTCTGGGCCATAGAACT-3′
Reverse 5′-CCACCACGCTCTTCTGTCTAC-3′
Actb
Forward 5′-CTCTCAGCTGTGGTGGTGAA-3′
Reverse 5′-AGCCATGTACGTAGCCATCC-3′

CLEAR, coordinated lysosome expression and regulation; Ctsd, cathepsin D; Lamp1, lysosome-associated membrane protein; Mcoln1, mucolipin 1 (TRPML1); Ppt1, palmitoyl-protein thioesterase 1; Tnfa, TNF-α; Actb, β-actin.