Fig. 2.

A: hypoxia increased glucose-6-phosphate dehydrogenase (G6PD) activity in CD133⁺ cells, but the effect was suppressed by dehydroepiandrosterone (DHEA) and 6-aminonicotinamide (6AN). Inhibition of G6PD with DHEA or 6AN also suppressed hypoxia-induced increases in cell number (B) and cell size (C) but slightly increased the incidence of necrotic cell death (D). Fluorescence micrographs showing CD133⁺ cells transfected with an adenoviral vector encoding green fluorescent protein (GFP) with G6PD-shRNA or GFP alone (E and F). G: G6PD activity was reduced in cells transfected with G6PD shRNA. H: transfection with G6PD shRNA also reduced CD133⁺ cell numbers.