Fig. 3.

CIS treatment after Mg deficiency is associated with enhanced neutrophil infiltration and renal myeloperoxidase (MPO). Mice were maintained on either 100% Mg or 10% Mg-deficient diets or were maintained on a 10% Mg diet followed by Mg supplementation, as described in methods, and then treated with saline (CTRL) or CIS (12 mg/kg). All mice were euthanized 48 h post-CIS (or saline); fixed kidney tissues were evaluated for neutrophils by Leder staining. A and B: representative images for each group at ×200 (A) and ×400 (B) magnification. C: mean numbers of neutrophils per high-power field (HPF) ± SE. D: frozen renal tissues were analyzed for MPO. Data are shown as mean MPO concentrations ± SE (in pg/mg protein). Scale bars = 20 μm. *P < 0.05 vs. CTRL; ††P < 0.01 vs. CIS; †††P < 0.001 vs. CIS; ‡‡‡P < 0.001 vs. MgD + CIS.