Fig. 4.

Mg deficiency before CIS treatment upregulates the expression of renal cytokines: reversal by Mg replacement. Mice were maintained on either 100% Mg or 10% Mg-deficient diets or were maintained on a 10% Mg diet followed by Mg supplementation, as described in methods, and then treated with saline (CTRL) or CIS (12 mg/kg). A: all mice were euthanized 48 h post-CIS (or saline), and renal TNF-α (Tnfa) mRNA expression in renal cortical tissues was measured using quantitative PCR. Data are shown as means ± SE (in fold changes vs. Gapdh). B and C: IL-6 (B) and IL-1β (C) renal protein levels were measured by MSD. Data are shown as mean cytokine concentrations ± SE (in pg/g protein). ††P < 0.01 vs. CIS; †††P < 0.001 vs. CIS; ‡‡P < 0.01 vs. MgD + CIS; ‡‡‡P < 0.001 vs. MgD + CIS.