Fig. 3.

Collagen content in the pancreas assessed by Sirius red staining after repetitive episodes of acute pancreatitis as described in Fig. 1. A: representative pancreatic sections of pancreas from saline treated control WT (a) and AT1a−/− (b) mice and from cerulein-treated WT (c) and AT1a−/− (d) mice. Collagen staining appears red; control mice demonstrate some perilobular collagen, and there was no difference between control WT and AT1a −/− mice. Cerulein-treated mice demonstrated extensive interlobular and periacinar collagen staining, indicating robust fibrogenesis in this model of chronic pancreatitis. Blinded analysis identified no difference between WT and AT1a −/− mice (original magnification ×200). B: relative amount of pancreatic collagen quantified by morphometric analysis demonstrates no differences between WT and AT1a −/− mice. Results are expressed as means ± SE, n = 6 mice in each group. *P < 0.01.