Fig. 6.

The effect of necrostatin-1 supplementation during vitrification in mouse oocytes. Oocytes from 10 to 14 weeks of age were vitrified and stored in LN₂ for 2–4 weeks. Necrostain-1 (Nec1, 1 μM) was added to the final vitrification solution (15% EG, 15% DMSO, and 0.5 M sucrose), as shown in (a). b The experiment was performed 5 times independently using the number of mice given in parentheses. The numbers of survived oocytes per total number of oocytes are shown within each bar. The values represent mean survival rates ± S.E.M. Statistical significance was analyzed by a two-tailed Student’s t-test. *, p = 0.0346, t = 2.542, df = 8