Fig. 1.

CYP1A1 is upregulated in PMs of septic mice. a Mice were intraperitoneally injected with vehicle (isopyknic PBS), LPS (20 mg/kg) or E. coli (1.2 × 10¹¹ CFUs/kg, CFUs, colony forming units). PMs were extracted at the indicated times and subjected to western blotting analysis of CYP1A1 protein levels. b PMs isolated from WT mice were treated with vehicle, LPS (10 μg/ml) or heat-killed E. coli (MOIs = 10, MOIs, multiplicity of infections) for the indicated times. CYP1A1 mRNA expression was quantified by qRT-PCR. Expression levels of CYP1A1 protein were detected by western blotting. c-e AhR^−/− and WT mice were intraperitoneally injected with vehicle or E.coli. After 12 h treatment, PMs and PLFs were extracted and subjected to analysis of AhR and CYP1A1 protein expression levels (a), pro-inflammatory cytokines expression levels (b) and PMs count (c). Data are mean ± SEM of three independent experiments. Results were compared by one-way ANOVA. *p < 0.05. NS, no statistical difference