TABLE 2.
Transfection conditions used for reverse genetics
| Plasmid function | Segment | Amt of plasmid (μg) under condition: |
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Original | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | ||
| Protein expression | PB2 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| PB1 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
| P3 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
| NP | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
| vRNA synthesis | PB2 | 0.2 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 |
| PB1 | 0.2 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.02 | 0.1 | 0.1 | 0.1 | 0.02 | 0.02 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.02 | |
| P3 | 0.2 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | |
| HEF | 0.2 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | |
| NP | 0.2 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | 0.2 | 1.0 | 0.6 | 0.2 | 0.2 | 0.5 | |
| M | 0.2 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | |
| NS | 0.2 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | 0.2 | 0.1 | 0.1 | 0.1 | 0.02 | 0.1 | |