FIG 2.

Nup358 depletion specifically affects adenoviral genome import. (A) U2OS cells were subjected to Nup358 depletion and viral infection using Ad5-wt-GFP viruses. The graph shows quantification of the capsid endosomal escape kinetic at the indicated time points, calculated as the number of Ad5 capsids positive for pVI relative to the total number of Ad5 capsids per cell in control (gray) versus KD Nup358 (blue) cells. Infections were carried out for 30 min at 37°C followed by inoculum removal (t = 0). Time point −15 min corresponds to 15 min before inoculum removal (n > 30 cells per condition/time point)*. (B) An experiment was performed as described in the Fig. 1D legend. Data represent results of quantification of capsid disassembly kinetic, calculated as the number of pVII-positive Ad5 capsids, relative to the total number of Ad5 capsids per cell (n > 30 cells per condition/time point)*. (C) An experiment was performed as described in the Fig. 1D legend. Data represent results of quantification of genome release kinetics from Ad5 capsids, calculated as the number of pVII-positive Ad5 capsids relative to the total number of detectable pVII per cell (n > 30 cells per condition/time point)*. *, the results of the experiment were analyzed using two-way ANOVA and Sidak’s multiple-comparison post hoc test.