FIG 2.

Reovirus strain T3A inhibits TNF-α-stimulated NF-κB-dependent gene expression. (A) ATCC L929 cells were treated with 10 ng/ml TNF-α. At 1 h following treatment, total RNA was extracted and subjected to RNA-seq analyses. A volcano plot showing genes whose expression is induced by 4-fold (log₂FC > 2) and which had an FDR of <0.05 in comparison to that in untreated cells are shown within the box. (B, C, D) ATCC L929 cells were adsorbed with 10 PFU/cell of T3A. Following incubation at 37°C for 20 h, cells were treated with 0 or 10 ng/ml TNF-α for 1 h. Total RNA was extracted from cells and was subjected to RNA-seq analyses. (B) Scatterplot comparing expression of the genes shown in the boxed region of panel A following infection with T3A with or without TNF-α. A trendline showing linear regression and coefficient of determination is shown. (C) Scatterplot comparing expression of the genes shown in the boxed region of panel A following TNF-α treatment of mock-infected and T3A-infected cells. A trendline showing linear regression and coefficient of determination is shown. (D) Heat map comparing expression of the genes shown in the boxed region of panel A following TNF-α treatment of mock-infected and T3A-infected cells. Expression of the same set of genes in T3A-infected cells is also shown.