FIG 5.

Reovirus gene expression is required for loss of IKKβ. (A) ATCC L929 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A in the presence or absence of 200 μM ribavirin. Following incubation at 37°C for 24 h, whole-cell extracts were immunoblotted with antisera specific for IKKβ, PSTAIR, and reovirus. (B) ATCC L929 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A in the presence or absence of 200 μM ribavirin. Following incubation at 37°C for 24 h, cells were treated with 10 ng/ml TNF-α and incubated for 1 h. Nuclear extracts were immunoblotted using antiserum specific for p65 or PSTAIR. (C) ATCC L929 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A in the presence or absence of 200 μM ribavirin. Following incubation at 37°C for 24 h, cells were treated with 10 ng/ml TNF-α and incubated for 1 h. RNA was extracted from cells, and IκBα gene expression was measured using RT-qPCR. Gene expression in mock-infected cells treated with TNF-α was set to 100%. Gene expression of each replicate, the mean value, and SD are shown. *, P < 0.05; NS, P > 0.05 by Student’s t test in comparison to mock-infected cells treated with TNF-α.