TABLE 3.
Amino acid difference between the reference and consensus sequences obtained in this study
| Cycle | Amino acid difference by positiona
(reference→substitution) |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VP3:501 (G→R), common | VP4:36 (P→L), common | VP4:180 (K→E), common | VP4:184 (V→A) |
VP4:187 (R→K), common | VP4:267 (C→Y), common | VP4:379 (T→I), common | VP6:245 (T→A), common | VP7:256 (E→G) |
|||||||
| a | b | c | d | a | b | c | d | ||||||||
| 0 | 100 | 100 | 100 | 33.4 | 33.4 | 2.8 | 2.8 | 100 | 100 | 100 | 100 | 0 | 0 | 0 | 0 |
| 1 | 100 | 100 | 100 | 25.2 | 60.3 | 13.5 | 14.4 | 100 | 100 | 100 | 100 | 0 | 0 | 2.6 | 0 |
| 2 | 100 | 100 | 100 | 44.1 | 68.4 | 39.4 | 69.0 | 100 | 100 | 100 | 100 | 0 | 0 | 34.2 | 0 |
| 3 | 100 | 100 | 100 | 70.5 | 77.4 | 64.0 | 80.5 | 100 | 100 | 100 | 100 | 0 | 0 | 70.4 | 0 |
| 4 | 100 | 100 | 100 | 79.5 | 76.4 | 73.8 | 87.0 | 100 | 100 | 100 | 100 | 0 | 0 | 72.0 | 0 |
| 5 | 100 | 100 | 100 | 86.4 | 83.1 | 83.6 | 89.0 | 100 | 100 | 100 | 100 | 0 | 0 | 96.2 | 0 |
a
Values show the frequency (%) of sequences possessing a substitution. a, 0.1MOI-1 lineage; b, 0.001MOI-1 lineage; c, 0.1MOI-2 lineage; d, 0.001MOI-2 lineage; common, common to all lineages.