FIG 9.
Long-term effects of the AEG compounds on the HIV-1 Env conformation. (A, B) VLPs prepared from A549 cells expressing HIV-1AD8 Env were incubated with DMSO or the indicated AEG compound and, in some cases, irradiated with UV light. The pelleted VLPs were washed and then resuspended in PBS with 2% DMSO. The VLPs were incubated at room temperature for 2 weeks. The VLPs were pelleted and lysed, and the cell lysates were incubated with the indicated antibodies and protein A-Sepharose. The precipitated proteins were analyzed by Western blotting with a rabbit anti-gp120 antiserum (top) and with the 4E10 anti-gp41 antibody (bottom). The results of typical experiments of two independent experiments are shown. Lanes M, molecular mass markers. The numbers to the left are molecular masses (in kilodaltons).