TABLE 1.
Primers used for qPCRa
| Gene | Accession no. | Primer sequence (5′–3′) | Reference |
|---|---|---|---|
| CLDN1 | XM_013108556.1 | F: TCATGGTATGGCAACAGAGTGG | 64 |
| R: TCATGGTATGGCAACAGAGTGG | |||
| IAV-M | F: CTTCTAACCGAGGTCGAAACG | 65 | |
| R: AGGGCATTTTGGACAAAGCGTCTA | |||
| IFIT5 | KF956064 | F: TCCTGCGATATGCTGCTATATTTTAT | 66 |
| R: GGTGTCACTGTTAAGGCTTTTCTCA | |||
| IFN-α | AB128861.1 | F: CAACGACACGCAGCAAGC | 40 |
| R: GGGTGTCGAAGAGGTGTTGG | |||
| IFN-β | KT428159 | F: TCTACAGAGCCTTGCCTGCAT | 67 |
| R: TGTCGGTGTCCAAAAGGATGT | |||
| IgA | U27222.1 | F: TCGCTCAAGGAACCCATCGT | 64 |
| R: GCGGGACCACGAGAACTTCA | |||
| GAPDH | AY436595.1 | F: CCACTTCCGGGGCACTGTCA | 63 |
| R: AGCACCAGCATCTGCCCACT | |||
| LYZ | XM_005008880.2 | F: TAACACGCAGGCTACAAACCG | 64 |
| R: TTCCATCGCTGACAATCCTCTT | |||
| MUC2 | XM_005024513.2 | F: GGGCGCTCAATTCAACATAAGTA | 64 |
| R: TAAACTGATGGCTTCTTATGCGG | |||
| OAS-L | KU569293.1 | F: CCGCCAAGCTGAAGAACCTG | 68 |
| R: CGCCCTGCTCCCAGGTATAG | |||
| Pan-Actinobacteria | F: TACGGCCGCAAGGCTA | 69 | |
| R: TCRTCCCCACCTTCCTCCG | |||
| Pan-Bacteroides | F: CRAACAGGATTAGATACCCT | 69 | |
| R: GGTAAGGTTCCTCGCGTAT | |||
| Pan-Firmicutes | F: TGAAACTYAAAGGAATTGACG | 69 | |
| R: ACCATGCACCACCTGTC | |||
| Pan-Fusobacteria | F: GCCTCACAGNTAGGGACAACAT | 69 | |
| R: GCYYACCTCTCCAGTACTCTAG | |||
| Pan-Gammaproteobacteria | F: TCGTCAGCTCGTGTYGTGA | 69 | |
| R: CGTAAGGGCCATGATG | |||
| POLRMT | XM_027472517.1 | F: CCACCAGAAGATCATGCAGC | 70 |
| R: CGATGGTCAGCAGCTTTCTC | |||
| RIG-I | NM_001310380 | F: GTGTATGGAGGAAAACCCTATTCTTAACT | 71 |
| R: GGAGGGTCATACCTGTTGTTTGAT | |||
| RPL4 | XM_027465643.1 | F: AAGCTGAACCCATACGCCAA | 63 |
| R: CCTGGGCCTTAGCTGTAACC | |||
| RPL30 | XM_027452502.1 | F: GCAAAGCCAAGCTGGTCATC | 63 |
| R: CTCAATGTTGTTGCCGCTGT | |||
| SDHA | XM_027451817.1 | F: GACACAGTGAAAGGCTCCGA | 63 |
| R: CTCCAGCTCTATCACGGCAG | |||
| TFAM | XM_013107580.1 | F: AGCATCACAGAAGCAGGTTTA | 70 |
| R: TGCCAGTCGTTTCCTCCT | |||
| USP18 | XM_005009931 | F: AACCTGACGGCAGAAGAAGA | 72 |
| R: GCACCGTGATCCTTCGTAGT | |||
| ZO-1 | XM_021276271.2 | F: TCAGCGAGATGAACGAGCC | 70 |
| R: TCTGAAGGCTCTGACCTCTGG |
a
CLDN1, Claudin-1; IAV-M: gene M of influenza A virus; IFIT5, interferon induced protein with tetratricopeptide repeats 5; IFN-α/β, interferons alpha and beta; IgA, immunoglobulin A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LYZ: lysozyme; MUC2, mucin 2; OAS-L, oligoadenylate synthetase-like; POLRMT, RNA polymerase mitochondrial; RIG-I, retinoic acid-inducible gene I; RPL4/30, ribosomal protein L4/L30; TFAM, mitochondrial transcription factor A; SDHA, succinate dehydrogenase complex flavoprotein subunit A; USP18, ubiquitin specific peptidase 18; ZO-1, zonula occludens-1. F, forward; R, reverse.