TABLE 4.

Antibody panel used in flow cytometry experiments to identify both magnitude and quality of CD8⁺ T cell response and relevant subpopulations^a

Antibody	Fluorochrome	Vol (ml)/test	Vendor	Catalog	Clone
CD4	APC ef 780	1	eBioscience	47-0049-42	RPA-T4
CD3	AF700	2	Biolegend	317340	OKT3
CD8	BV650	2	BioLegend	301042	RPA-T8
CD14	V500	2	BD Biosciences	561391	M5E2
CD19	V500	2	BD Biosciences	561121	HIB19
Fixability dye	ef506	1 μl/ml of master mix	eBioscience	65-0866-18	NA
IFN-γ	FITC	1	eBioscience	11-7319-82	4S.B3
CD154 (CD40L)	PE	2	eBioscience	12-1548-42	24-31
CD69	PE Cy7	2	eBioscience	25-0699-42	FN50
CD137(4-1BB)	APC	2	BioLegend	309810	4B4-1

^aAbbreviations: APC, allophycocyanin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; NA, not applicable.