FIG 7.

(A) Western blot analysis of IκBα in either TNF-α-stimulated ST cells (100 ng/ml; left), PRV Kaplan-infected ST cells challenged at 4 hpi with TNF-α (MOI, 10 PFU/cell; 100 ng/ml; middle), or PRV Kaplan-infected ST cells that were left untreated (MOI, 10 PFU/cell; right), analyzed at the indicated time points. (B) Confocal microscopy of NF-κB p65 in mock-infected or PRV-infected ST cells (4 hpi; MOI, 10 PFU/cell) treated or not treated with TNF-α for 30 min (100 ng/ml). NF-κB p65 is shown in green, PRV gB is shown in red, and nuclei are shown in blue. Bar, 50 μm. (C) Western blot analysis of total and Ser³²-phosphorylated IκBα protein in either mock-infected or PRV-infected cells (4 hpi; MOI, 10 PFU/cell) treated or not treated with TNF-α for 5 min (100 ng/ml). The Western blots and the confocal microscopy images shown are representative examples from three independent experimental repeats.