FIG 5.

Regulation of FMDV replication by hnRNP L is due to its binding to viral IRES. (A) Schematic representation of the IRES-replaced mutant FMDV(EMCV). FMDV genome showing the encoded polyproteins (P1, P2, and P3) flanked by the 5′ UTR and 3′ UTR. In the mutant FMDV(EMCV) genome, the complete IRES of FMDV was replaced by that of EMCV. The cognate IRES of FMDV(WT) (green) was exchanged with its counterpart from EMCV (yellow). (B) RNA-protein pulldown assay between EMCV IRES and hnRNP L. (C) BHK-21 cells or L3 clones were infected with FMDV(WT) and FMDV(EMCV). The resulting viruses were harvested at 4 h, 8 h, and 12 h, and the supernatant was analyzed for viral protein expression by Western blotting. (D) Schematic diagram of hnRNP L binding to FMDV IRES.