TABLE 4.
Primers for constructing EBNA3A mutants
| Primer | Sequence | Purpose |
|---|---|---|
| EBNA-3A-stop P1 | GGTGTTGGTGAGTCACACTTTTGTTGCAGACAAAATGGACTAGGATAGGCCGGGTCCCCCGGCCCTAGGATGACGACGATAAGTAGGG | ΔEBNA3A mutant |
| EBNA-3A-stop P2 | CTTCTTCCATGTTGTCATCCAGGGCCGGGGGACCCGGCCTATCCTAGTCCATTTTGTCTGCAACAACAACCAATTAACCAATTCTGATTAG | ΔEBNA3A mutant |
| EBNA-3A-Rev.WT-Pr1 | GGTGTTGGTGAGTCACACTTTTGTTGCAGACAAAATGGACAAGGACAGGCCGGGTCCCCCGGCCCTAGGATGACGACGATAAGTAGGG | ΔEBNA3A revertant |
| EBNA-3A-Rev.WT-Pr2 | CTTCTTCCATGTTGTCATCCAGGGCCGGGGGACCCGGCCTGTCCTTGTCCATTTTGTCTGCAACAACAACCAATTAACCAATTCTGATTAG | ΔEBNA3A revertant |
| EBNA3A-Stp-Chk-Fwd | TCCGGTGGTGACGTTAATTG | PCR and sequencing |
| EBNA3A-Stp-Chk-Rev | ACTTAGGCCATAGGGATGCT | PCR and sequencing |
| Cam-Ff-Kan-Fwd | CGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGAGCCATATTCAACGGGAAAC | Swap chloramphenicol with kanamycin in F-factor |
| Cam-Ff-Kan-Rev | CAGGCGTAGCAACCAGGCGTTTAAGGGCACCAATAACTGCCTTAAAAAAATTAGAAAAACTCATCGAGCATC | Swap chloramphenicol with kanamycin in F-factor |
| 2089-CamR-Fwd | CGGGCGTATTTTTTGAGTTATCG | Swap kanamycin with chloramphenicol in F-factor |
| 2089-CamR-Rev | CAGGCGTAGCAACCAGGCG | Swap kanamycin with chloramphenicol in F-factor |