TABLE 6.

Primers used for site-directed mutagenesis

Primer	Sequence	Purpose
First ATG mutant Fwda	GCGGCCGTCTCCTTTAAGACAAATCGGACAAGGACAG	Mutating the 1st ATG
First ATG mutant Reva	CTGTCCTTGTCCGATTTGTCTTAAAGGAGACGGCCG	Mutating the 1st ATG
Second ATG mutant Fwd	ATGGGACTTCTTCTTCCGAGTTCTCATCCAGGGCCG	Mutating the 2nd ATG
Second ATG mutant Rev	CGGCCCTGGATGACAACTCGGAAGAAGAAGTTCCAT	Mutating the 2nd ATG
EBNA3A mutant nucleotide Fwd	GGGGGACCCGGCCTATCCTAGTCCATTTTGTCTTAAAG	Mutating nucleotides in Bac mutantb
EBNA3A mutant nucleotide Rev	CTTTAAGACAAAATGGACTAGGATAGGCCGGGTCCCCC	Mutating nucleotides in Bac mutant
EBNA3A mutant deletion Fwd	GGGGACCCGGCCTATCTAGTCCATTTTGTCTT	Adding deletion in Bac mutant
EBNA3A mutant deletion Fwd	AAGACAAAATGGACTAGATAGGCCGGGTCCCC	Adding deletion in Bac mutant

^aPrimers were used to generate the EBNA3A site-directed mutant used to determine if EBNA3A can be expressed from the second ATG.

^bBACmid (Bac) mutant.