TABLE 2.

NS5B polymerase mutations associated with sofosbuvir resistance^a

Rat ID and day p.i.	Plasma viral load (genomes/ml)b	Substitution
		Nucleotidec	Amino acidd
R352e
2	3.35 × 108
10	<LOD	Not determined	Not determined
14	9.95 × 106	T8445A	S2654T
R362
2	2.64 × 107
10	<LOD	Not determined	Not determined
14	2.84 × 105	T8445A	S2654T
21	9.35 × 109

^aBoldface indicates sofosbuvir treatment.

^bLimit of detection (LOD) of RT-PCR assay is 1,875 genomes/ml.

^cNucleotide position according to full-length RHV genome (accession no. KX905133).

^dAmino acid position according to the start of the RHV polyprotein (accession no. AQV09561; protein cleavage sites predicted in reference 10).

^eRat R352 was euthanized at day 14 prior to treatment cessation and virus rebound.