TABLE 1.
Primers for the amplification of p50, p65, and Keap1 genesa
| Primer | Sequence (5′-3′) |
|---|---|
| P50-F1 | TATACAGCTCCAGAATGGC |
| P50-R1 | CACGTCTTCCTGCTTAGTG |
| P50-F2 | TTTTGGATCCATGGCAGACGACGACC |
| P50-R2 | TTTCTCGAGATGGTTCATCCCAGC |
| P65-F1 | ATTTCCGCCTCTGGCGAATG |
| P65-R1 | CAAACGCTGGTGTTAGGC |
| P65-F2 | TTTGGATCCATGGACGAACTGTTCC |
| P65-R2 | TTTTCTCGAGGGAGCTGATCTGACTC |
| Keap1-F1 | GCGTGATCCCTTGTCACTT |
| Keap1-R1 | CACGTCAACAGGTGCAGTT |
| Keap1-F2 | TTTTGGATCCATGCAGCCGGAAGCC |
| Keap1-R2 | TTTCTCGAGTCAACAGGTGCAGTTC |
a
Restriction site sequences are underlined. GGATCC, BamH I; CTCGAG, Xho I.