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. Author manuscript; available in PMC: 2021 Feb 5.

Published in final edited form as: J Am Soc Mass Spectrom. 2020 Jan 13;31(2):394–404. doi: 10.1021/jasms.9b00024

Table 5.

Mass List of Accurate Mass of Signature Fragment Ions Generated Using Various Biotinylation Reagents

experiment	Δ mass (Da) for db searches	signature fragment ions (m/z)
		dehydrobiotin	ImKbio	ImKbio-NH3
biotin	226.078	227.085	327.185	310.158
heavy biotin	230.031	231.110	331.210	314.184
SILAC K4 + biotin	230.031	227.085	331.210	314.184
SILAC K6 + biotin	232.013	227.085	333.210	316.172
SILAC K8 + biotin	234.092	227.085	334.196	318.168
		dyhydrobiotin	ImYBxxp	ImYBxxp-NH₃
biotin-XX-phenol	587.314	227.085	723.388	706.361
		biotin-XX	biotin-X
		453.251	340.168