In vivo imaging of the PCCA in rat liver during minimum respiratory motion using the UIAU imaging technique. (a) Pre-activation B-mode image with superimposed activation beam focal locations. The overlap region between the liver and the activation foci, where the baseline, pre-activation and post-activation PCCAs signal magnitudes are calculated, is indicated by the green boundary. The region outside the PCCA activation zone, where the signal magnitude of the echogenic clutter is calculated, is indicated by the yellow rectangle boundary. (b) Baseline UIAU image of the liver before the PCCAs are injected; (c) Pre-activation UIAU image of the PCCA with the activation pulses turned off; (d) UIAU image of the vaporized PCCA; (e) PCCA image in (d), shown in green, overlaid on the B-mode image (a) (grayscale); Baseline and pre-activation images are all normalized to the post-activation images. All images are log-compressed and displayed with 30 dB dynamic range.