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. 2020 May 4;40(5):BSR20192743. doi: 10.1042/BSR20192743

Figure 3. PTEN was identified as a target for miR-92a-3p.

Figure 3

To further investigate the mechanisms underlying the positive effects of miR-92a-3p on SCI progression, we screened the possible target genes for miR-92a-3p by TargetScan. (A) A possible binding site of miR-92a-3p was identified in the 3′-UTR of PTEN. (B) The dual-luciferase reporter assay was performed to verify the association between PTEN and miR-92a-3p. **P<0.01 vs. Blank. (C–E) We further measured the expressions of PTEN in BV-2 cells transfected with miR-92a-3p mimic by qRT-PCR and Western blot. Each value represents mean ± SD. GAPDH served as an internal control. **P<0.01 vs. mimic control. Abbreviation: qRT-PCR, quantitative real-time RT-PCR.