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. Author manuscript; available in PMC: 2020 May 5.
Published in final edited form as: Curr Biol. 2019 Oct 3;29(20):3359–3369.e4. doi: 10.1016/j.cub.2019.08.003

Figure 2. Abrogation of OGT in the Adult Brain Elicits Aging-Associated Neuronal Phenotypes in the Hippocampus.

Figure 2.

(A) Schematic depicting the timeline for the generation of and subsequent experiments using the conditional excitatory neuron Ogt knockout (Ogt-cKO) and control mouse. See also Figure S2.

(B) Representative images of phosphorylated CREB (pCREB) and cFos immunostain and Nissl stain in the hippocampus of young Ogt-cKO and control mice. Scale bar, 200 mm. See also Figure S2.

(C) Quantification of pCREB immunostain mean intensity in CA1 and DG regions of the hippocampus, n = 4 mice per group.

(D) Quantification of cFos-positive cells in the CA1 and DG regions of the hippocampus, n = 4 mice per group.

(E) Quantification of Nissl stain mean intensity in CA1 and dentate gyrus (DG) regions of the hippocampus, n = 3 Ogt-cKO and 4 control mice. See also Figure S2.

(F) Representative field of Golgi stain from CA1 pyramidal neurons and DG granule cell neurons in young Ogt-cKO and control mice.

(G) Quantification of spine number from tertiary dendrites of CA1 pyramidal neurons and DG granule cell neurons in Ogt-cKO and control mice; n = 10 neurons per mouse and 5 mice per group. Data are represented as mean ± SEM. *p < 0.05; **p < 0.01; t test (C–E and G).