TABLE 1.

Rationalization of CA_D variants.

Constructs	Residues
1QRG (Cam)	R59	E62	Q75	E84	N202	H81	H117	H122
CA_D	R43	I46	K58	D67	H166	H64	H89	H94
I46E	R43	E46	K58	D67	H166	H64	H89	H94
K58Q	R43	I46	Q58	D67	H166	H64	H89	H94
H166N	R43	I46	K58	D67	N166	H64	H89	H94
I46E-K58Q	R43	E46	Q58	D67	H166	H64	H89	H94
K58Q-H166N	R43	I46	Q58	D67	N166	H64	H89	H94
I46E-H166N	R43	E46	K58	D67	N166	H64	H89	H94
CA_D* (I46E-K58Q-H166N)	R43	E46	Q58	D67	N166	H64	H89	H94
CA_D*-D67E	R43	E46	Q58	E67	N166	H64	H89	H94

The residues in bold were modified based on the alignment with Cam shown in the first row.