Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 9;2020:9207279. doi: 10.1155/2020/9207279

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Ananda S. Prasad.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Activation of TCR increases intracellular free zinc. (a) Cells were incubated for 24 h in normal media to which had been added 15 μM zinc (to load cells with zinc) and then stimulated in normal media (5 μM zinc) in the presence of PHA, PMA, or Con-A for 30 and 60 min to determine which pathway was involved in differentiation or activation of Th0 or naïve cells and resulted in an increase in cellular free zinc. (b) To determine the time course for an increase in cellular free zinc, HUT-78 cells were stimulated in normal or in zinc-deficient medium. Stimulation in normal vs. zinc-deficient media allowed us to determine if the increase in cellular free zinc was due to an influx of extracellular free zinc or only a release of free zinc within the cellular compartment under Con-A stimulation. It appears that the increase in cellular free zinc is a result of both extracellular zinc influx and the release of free zinc from intracellular sources following Con-A stimulation (n = 3) [37].