Figure 2.

Impacts of each pr1 deletion on transcriptional expression of 10 other pr1 genes and total levels of intra- and extracellular Pr1 activities in B. bassiana. (a) Relative transcript levels of all pr1 genes in the cDNA samples of given pr1 mutants with respect to the WT standard. All cDNA samples were derived from the submerged cultures of a 10⁷ conidia/ml suspension in CDB-BSA incubated for 30 h at 25°C. Note that each deleted pr1 gene was transcriptionally undetectable in the corresponding Δpr1 mutant. (b–d) Biomass levels, intracellular Pr1 activities, and extracellular Pr1 activities (EPA) quantified from the 30-h-old CDB-BSA cultures of all Δpr1 mutants and control strains (WT and complemented strains), the protein extracts of their cultures and the supernatants of their cultures, respectively. Note no significant variability among measurements of all tested strains in (b) and (c). The asterisked Δpr1 means in (d) differ significantly from those of the corresponding control strains unmarked (Tukey’s HSD, P < 0.05). Error bars: SD from three replicates.