Figure 3.

Impacts of each pr1 deletion on germination of conidia and secretion of Pr1 proteases in the presence of insect cuticle. (a) GT₅₀ (h) estimates of all tested strains for 50% conidial germination on agar plates and locust hindwings at optimal 25°C. (b) Total levels of extracellular Pr1 activities (EPA) quantified from the supernatants of the submerged cultures incubated 24 and 36 h at 25°C in 0.625% M-100 salt solution containing 1% ground locust cuticle as a sole nutrient source. Each culture was generated from the shaking incubation of a 10⁶ conidia/ml suspension in the cuticle broth. The asterisked Δpr1 means in each bar chart differs significantly from those of the corresponding control strains unmarked (Tukey’s HSD, P < 0.05). Error bars: SD from three replicates.