FIGURE 6.

IL-21 and IL-4 production by Tfh cells in C. rodentium infection. We orally inoculated 3- to 4-wk-old mice with a low dose of C. rodentium. We sacrificed the mice at day 9 postinfection to see the immune responses in MLNs and spleens. Flow cytometric analysis of IgG1⁺ GC B cells (A), IgG2a⁺ GC B cells (B), IgG2b⁺ GC B cells (C), IL-21⁺ Tfh cells (D), IL-4⁺ Tfh cells (E), and IFN-γ⁺ Tfh cells (F) in MLNs and spleens are shown. We calculated the cell numbers. Each point represents an individual mouse. Data are shown as mean ± SEM and derived from two independent experiments. *p ≤ 0.05, **p ≤ 0.01, t test.