Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 19;219(5):e201903181. doi: 10.1083/jcb.201903181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Yaniv et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — The linear actin elongation factor Ena, but not the formins DAAM or Frl, is required for developmental axon regrowth. (A–C) Confocal z-projections of WT (A), ena^Δ151 (B), or ena^Δ151 additionally expressing UAS-Ena (C), γ-neuron adult neuroblast MARCM clones. (D and E) Confocal z-projections of adult MARCM neuroblast clones of ena^Δ151 additionally expressing UAS-chic (E) or chic^Δ29-33 neuroblast clones additionally expressing UAS-ena (D). (F) Box plot quantification of MB γ axon regrowth of A (n = 15), B (n = 10), D (n = 14), E (n = 11), and Fig. 1 I (n = 12). ANOVA analysis with Tukey's honestly significant difference (HSD) post hoc was performed. ena^Δ151 with and without UAS-Chic, ***, P < 0.001; chic^Δ29-33 with and without UAS-Ena, P = 0.99. (G–I) Confocal z-projections of DAAM^ΔORF (G), Frl ^ΔORF (H), or double mutants for DAAM^ΔORF and Frl ^ΔORF (I) γ-neuron adult neuroblast MARCM clones. (J) Box plot quantification of MB γ axon regrowth of A (n = 15), B (n = 10), C (n = 18), G (n = 11), H (n = 9), and I (n = 7); as well as Fig. S2 C (n = 5), Fig. S2 D (n = 15), and Fig. S2 E (n = 10). ANOVA analysis with Tukey's HSD post hoc was performed. Unless specifically marked otherwise, the significance level that is indicated above the box is in comparison to the WT. P values, compared with WT, are as follows: ena²³, ***, P = 0.002; ena²¹⁰, ***, P < 0.001; ena^LL03568, ***, P < 0.001; ena^Δ151, ***, P < 0.001; DAAM^ΔORF, P = 0.999; Frl^ΔORF, P = 0.947; double mutants for DAAM^ΔORF and Frl^ΔORF, P = 0.997. ena^Δ151 with and without UAS-Ena, ***, P < 0.001. In all box plots, boxes encompass the values in between the first and third quartiles; whiskers are ± 1.5 IQR; median (line), mean (blue triangle), and outliers (red diamond). In all confocal images, asterisks mark the distal tip of the adult γ lobe. Green is R71G10-Gal4–driven mCD8::GFP. Magenta represents FasII staining. Scale bars, 20 µm. n.s., not significant.