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. 2020 Mar 19;219(5):e201903181. doi: 10.1083/jcb.201903181

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© 2020 Yaniv et al.

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Figure S3. — Description of formin CRISPR/Cas9 alleles and additional formin phenotypes. (A and B) Scheme depicting the genomic locus of DAAM (A) or Frl (B) with the CRISPR/Cas9 mediated deletions (ΔORF) annotated as dashed lines. Red and gray bars depict coding and noncoding exons, respectively, while lines depict introns. (C–E) Confocal z-projections of DAAM^ΔORF (C), Frl ^ΔORF (D), or DAAM^ΔORF/Frl^ΔORF (E) MB γ-neuron MARCM clones at third instar larva (L3). (F) Confocal z-projections of dia^Δ33-130 adult γ-neuron MARCM neuroblast clone. (G) Scheme depicting the genomic locus of dia with the CRISPR/Cas9 mediated indel. Gray and green are 71G10-Gal4 (C, D, and G) or 201Y-Gal4 (E)–driven mCD8::GFP. Magenta is FasII staining. Scale bars, 20 µm.