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. 2020 Mar 19;219(5):e201903181. doi: 10.1083/jcb.201903181

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© 2020 Yaniv et al.

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Figure S4. — The Arp2/3 complex is not required for developmental axon regrowth of MB γ-neurons. (A) Heatmap depicting the relative RNA expression levels of the Arp2/3 complex members that are expressed above threshold throughout development from second instar larva (L2) to adult. Genes are ordered by their relative abundance during their peak expression as depicted by the purple heatmap on the right. See Alyagor et al. (2018) for technical details. (B) Scheme depicting the genomic locus of Arp3. arp3^Δ30-33 is a four-nucleotide deletion starting 30 bp downstream of the start site causing a frameshift after nine amino acids. Red and gray bars depict coding and noncoding exons, respectively, while lines depict introns. (C–H) Confocal z-projections of WT (C), arp3^Δ30-33 (D), arpC4^SH1036 (E), arpc1^Q25st (F), arpc1^R337st (G), and SCAR^Δ37 (H) adult γ-neuron MARCM neuroblast clones. Green is R71G10-Gal4–driven mCD8::GFP. Magenta represents FasII staining. Scale bars, 20 µm.