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. 2020 Apr 9;219(5):e201908179. doi: 10.1083/jcb.201908179

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© 2020 Penfield et al.

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Figure 3. — CNEP-1 promotes nuclear closure after anaphase of meiosis II. (A) Single z-slices of confocal and magnified images of oocyte-derived pronuclei expressing GFP::SP12 (ER marker) from time lapse series of indicated conditions. (B) Plot of percent of oocyte-derived (O) and sperm-derived (S) pronuclei with minor or major internal nuclear membranes with minor as defined in magnified image examples. (C) Confocal images of oocyte-derived pronuclei in indicated conditions. (D) Plot of average ± SEM of nuclear:cytoplasmic ratio of GFP::NLS-LacI for indicated conditions. n = no. of embryos. Scale bars, 2.5 µm.