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. 2020 Apr 9;219(5):e201908179. doi: 10.1083/jcb.201908179

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© 2020 Penfield et al.

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Figure 4. — Loss of NE-adaptors for ESCRTs exacerbates nuclear defects in meiotic and mitotic cnep-1 mutants. (A) Top: Schematic of pronuclear positioning and NE breakdown in the C. elegans zygote relative to pseudocleavage (PC) regression. Bottom: Confocal images of oocytes from time lapse series of embryos for the indicated conditions. Arrowheads mark the oocyte-derived pronucleus. (B) Plot of nuclear entry of GFP::α-tubulin under indicated conditions. P values reported from a Mann–Whitney test. (C) Confocal images of two-cell embryos 240 s before the following anaphase of the P1 cell. (D) Plot of percentage of nuclear entry of GFP::α-tubulin under indicated conditions. n = no. of cells. (E) Plot of percentage of embryos with indicated nuclear phenotypes. n = no. of embryos. Scale bars, 5 µm.