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LEM-2 plaque forms and disperses as the spindle dissipates and detaches from the oocyte-derived pronucleus, related to Fig. 1. C. elegans oocyte in meiosis II expressing GFP::LEM-2 and mCherry::α-tubulin. Arrows mark initial GFP::LEM-2 rim and the formation of the GFP::LEM-2 plaque. The video starts at −100 s before maximal spindle shortening and mages were acquired every 20 s, and the playback rate is 60× real time. Scale bar, 10 µm.
