Figure S2.

syp mutants created by CRISPR method in this study. (A) For syp-5 gene editing, syp-5(cac1) (asterisk) is an Indel frameshift mutation that occurs downstream of the initiation codon within the first exon and creates a putative 16-aa protein product. Thus, this mutant was considered to be a null mutant, which was also confirmed by SYP-5 immunostaining in Fig. S5 B. syp-5::gfp (cac4) (black arrowhead) was created by inserting the coding sequences of the 4-aa linker “GGSG” and the GFP protein before the stop codon. In syp-5(delC)(cac27) mutant allele, a 1,397-bp genome sequence (red line) was deleted to remove a SYP-5 C-terminal IDR region that contains a CIE cluster. In the syp-5(syp-6 C)(cac34) mutant, a 2,512-bp syp-5 genomic sequence (green line) that encodes the C-terminal 162-aa region was replaced by a 1,160-bp syp-6 genomic sequence that encodes the SYP-6 C-terminal 291-aa region. In the syp-5(14K)(cac43) mutant, point mutations for 14 aa within the SYP-5 C-terminal region were introduced into the syp-5 genome sequence (within the blue line region). (B) For syp-6 gene editing, syp-6(cac3) contained a 2,068-bp deletion mutation, removing the majority of the gene sequence (red line) and resulting in a frameshift of the remaining coding sequence, and this mutant was thus considered a null mutant. syp-6(cac5) (black arrowhead) was created by inserting the coding sequences of the 4-aa linker “GGSG” and GFP protein before the stop codon. Green line indicates the region used to replace syp-5 sequence in the syp-5(syp-6 C) mutant. (C) For the syp-4(22K)(cac42) mutant, point mutations of 22 aa within the SYP-4 C-terminal IDR region were introduced into a 544-bp region in the syp-4 genome sequence (blue line). (D) Plate phenotype analysis for the listed genotypes.