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. 2020 Mar 30;219(5):e201907079. doi: 10.1083/jcb.201907079

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© 2020 Sakakibara et al.

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Figure S1. — Requirement of afadin for the proper actin organization at AJs in low and high magnifications. Localization of E-cadherin and F-actin in wild-type and afadin-KO cells. Wild-type and afadin-KO cells were fixed. The samples were stained with the indicated Abs and phalloidin, followed by immunofluorescence microscopic analysis. The boxed regions in the upper panels are magnified in the lower panels. Arrows indicate the F-actin signal at bicellular AJs. The results are representative of three independent experiments.