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. 2020 Apr 7;219(5):e201908099. doi: 10.1083/jcb.201908099

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© 2020 Spriggs et al.

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Figure S1. — BICD1, but not BICDR2, supports SV40 infection (related to Fig. 1). (A) CV-1 cells were transfected with 5 nM of the indicated siRNA for 48 h. RNA was isolated and RT-PCR used to assess the transcript levels. GAPDH was used as a loading control. (B) CV-1 cells were transfected with 5 nM of the indicated siRNA and infected with SV40 (MOI ∼1). At 24 hpi, cells were fixed and stained for TAg. Data were normalized to the scrambled control. Values are the averages of the means (n = 3) ± SD. A standard Student’s t test was used to determine statistical significance. **, P ≤ 0.005; ***, P ≤ 0.0005.