Figure S4.

The SV40-induced ER focus is surrounded by BICD2 and BICDR1 (related to Fig. 6). (A) Confocal analysis of CV-1 cells that were infected with SV40 (MOI ∼5) for 16 h and stained with anti-VP1 (green) and anti-BICD2 (red) antibodies. Cells were counterstained with DAPI (blue). (B) Confocal analysis of uninfected and SV40-infected cells (MOI ∼5) stained with anti-VP2/3 (pink), anti-BAP31 (green), and anti-BICD2 (red) antibodies. Cells were counterstained with DAPI (blue). (C) Confocal analysis of control and BICDR1-depleted CV-1 cells infected with SV40 (MOI ∼5). At 16 hpi, cells were stained with anti-VP2/3 (green), anti-BAP31 (red), and counterstained with DAPI (blue). The graph represents the percentage of cells with BAP31⁺ foci normalized to the scrambled control. (D) As in A, except stained with anti-GM130 (green) and anti-BICDR1 (red) antibodies. Cells were counterstained with DAPI (blue). Scale bars, 10 µm. Values are averages of the means (n = 3) ± SD. A standard Student’s t test was used to determine statistical significance.