Table 1.

Experimental diets’ ingredients and chemical composition (as-fed basis)

	0% CA				0.3% CA
	dEB, mEq/kg				dEB, mEq/kg
Ingredient	−50	100	250	400	−50	100	250	400
Ingredients,%
Corn	66.00	66.12	64.14	61.48	65.70	65.82	63.84	61.18
Soybean meal	6.33	6.31	6.72	7.27	6.33	6.31	6.72	7.27
Soy–protein concentrates	3.00	3.00	3.00	3.00	3.00	3.00	3.00	3.00
Whey	10.00	10.00	10.00	10.00	10.00	10.00	10.00	10.00
Fish meal	5.00	5.00	5.00	5.00	5.00	5.00	5.00	5.00
Spray-dried plasma protein	5.00	5.00	5.00	5.00	5.00	5.00	5.00	5.00
Limestone	0.06	0.88	1.07	1.06	0.06	0.88	1.07	1.06
Dicalcium phosphate	0.40	0.40	0.41	0.42	0.40	0.40	0.41	0.42
CA	0.00	0.00	0.00	0.00	0.30	0.30	0.30	0.30
Chromic oxide	0.30	0.30	0.30	0.30	0.30	0.30	0.30	0.30
l-Lys•HCl (98%)	0.53	0.53	0.52	0.51	0.53	0.53	0.52	0.51
dl-Met	0.11	0.11	0.11	0.11	0.11	0.11	0.11	0.11
l-Thr	0.13	0.13	0.13	0.13	0.13	0.13	0.13	0.13
l-Trp	0.05	0.05	0.05	0.04	0.05	0.05	0.05	0.04
Soybean oil	1.30	1.27	1.90	2.75	1.30	1.27	1.90	2.75
Calcium chloride	1.08	0.21	0.00	0.00	1.08	0.21	0.00	0.00
Sodium bicarbonate	0.00	0.00	0.96	2.23	0.00	0.00	0.96	2.23
Choline chloride (50%)	0.10	0.10	0.10	0.10	0.10	0.10	0.10	0.10
Antioxidant	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05
Vitamin and mineral premix1	0.85	0.85	0.85	0.85	0.85	0.85	0.85	0.85
Calculated composition
CP, %	18.00	18.00	18.00	18.00	18.00	18.00	18.00	18.00
ME3, kcal/kg	3,350	3,350	3,350	3,350	3,350	3,350	3,350	3,350
Calcium, %	0.80	0.80	0.80	0.80	0.80	0.80	0.80	0.80
Phosphorus, %	0.36	0.36	0.36	0.36	0.36	0.36	0.36	0.36
Lys2, %	1.35	1.35	1.35	1.35	1.35	1.35	1.35	1.35
MET2,%	0.39	0.39	0.39	0.40	0.39	0.39	0.39	0.40
Met+Cys2,%	0.74	0.74	0.74	0.74	0.74	0.74	0.74	0.74
Thr2,%	0.79	0.79	0.79	0.79	0.79	0.79	0.79	0.79
Trp2,%	0.22	0.22	0.22	0.22	0.22	0.22	0.22	0.22
Analyzed composition
Na,%	0.17	0.18	0.44	0.78	0.16	0.18	0.41	0.82
K,%	0.65	0.65	0.65	0.65	0.57	0.67	0.70	0.66
Cl,%	1.05	0.52	0.39	0.38	0.95	0.55	0.40	0.43
dEB3, mEq/kg	-55.99	97.86	247.62	398.33	-52.59	94.51	244.54	404.19

¹Vitamin-mineral premix supplied per kilogram of feed: 10,000 IU of vitamin A, 100 IU of vitamin D3, 80 IU of vitamin E, 2.0 mg of vitamin K3, 0.03 mg of vitamin B12, 12 mg of riboflavin, 40 mg of niacin, 25 mg of d-pantothenic acid, 0.25 mg of biotin, 1.6 mg of folic acid, 3.0 mg of thiamine, 2.25 mg of pyridoxine, 300 mg of choline chloride, 150 mg of Fe (FeSO₄), 100 mg of Zn (ZnSO₄), 30 mg of Mn (MnSO₄), 25 mg of Cu (CuSO₄), 0.5 mg of I (KIO₃), 0.3 mg of Co (CoSO₄), 0.3 mg of Se (Na₂SeO₃), and 0.4 mg of ethoxyquin.

²Standardized ileal-digestible.

³dEB was calculated using the following equation: dEB (mEq/kg) = [(Na × 434.98) + (K × 255.74) − (Cl × 282.06)].