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. 2020 May 5;98(5):skaa116. doi: 10.1093/jas/skaa116

Table 1.

Ingredients and chemical composition of the experimental diets (± SD; n = 4 per diet)

Control AFEX1
Item Enz− 2 Enz+ 3 Enz− Enz+
Ingredient, % DM
 Alfalfa pellets4 50.0 20.0
 AFEX straw pellets5 30.0
 Barley grain 35.3 35.3
 Corn Dried distillers’ grain with solubles (DDGS)6 5.0 5.0
 Canola meal 5.0 5.0
 Dried molasses 1.3 1.3
 Canola oil 1.0 1.0
 Sheep mineral6 1.0 1.0
 Urea 0.5 0.5
 Ammonium chloride 0.4 0.4
 Dicalcium phosphate 0.3 0.3
 Calcium carbonate 0.250 0.250
 Vitamin ADE 0.020 0.020
 Bovatec 20 (20% lasalocid) 0.018 0.018
Chemical composition
 DM, % 95.9 ± 0.71 95.9 ± 0.74 96.3 ± 0.62 96.4 ± 0.55
 OM, % of DM 91.5 ± 0.33 91.5 ± 0.63 93.2 ± 0.36 93.4 ± 0.40
 CP, % of DM 19.6 ± 0.94 19.7 ± 0.97 16.5 ± 0.16 17.0 ± 0.56
 NDF, % of DM 30.6 ± 3.85 30.5 ± 2.90 38.0 ± 1.45 36.9 ± 2.06
 ADF, % of DM 21.8 ± 3.24 22.3 ± 3.61 27.9 ± 1.64 28.5 ± 1.19
 NFC, % of DM 37.7 ± 2.87 37.7 ± 2.49 35.8 ± 1.15 36.6 ± 1.70
 Ether extract, % of DM 3.7 ± 0.28 3.7 ± 0.47 2.9 ± 0.23 3.0 ± 0.16
 Ca, % of DM 1.15 ± 0.069 1.14 ± 0.132 0.64 ± 0.087 0.66 ± 0.061
 P, % of DM 0.41 ± 0.042 0.43 ± 0.040 0.37 ± 0.029 0.37 ± 0.044
 NEm, Mcal/kg 1.61 ± 0.062 1.60 ± 0.066 1.52± 0.025 1.55 ± 0.029
 NEg, Mcal/kg 1.00 ± 0.057 1.00 ± 0.055 0.93 ± 0.021 0.95 ± 0.028

1AFEX = ammonia fiber expansion treatment (30% AFEX straw pellets on a dietary DM basis replacing alfalfa hay pellets).

2Enz− = diet without enzyme.

3Enz+ = diet with enzyme.

4Chemical composition of alfalfa hay pellets: 89.0% OM, 17.8% CP, 54.8% NDF, 44.5% ADF, and 2.0% ether extract.

5Chemical composition of AFEX wheat straw pellets: 94.2% OM, 9.7% CP, 69.9% NDF, 54.4% ADF and 0.9% ether extract.

6Sheep mineral constituents (%): salt 92.6, potassium magnesium sulfate 4.979, zinc sulfate 0.921, magnesium sulfate 0.835, organic iodine 0.014, 1% selenium premix 0.143, cobalt carbonate 0.004, canola oil 0.398. Vitamin ADE constituents: vitamin A 10,000,000 IU, vitamin D 1,000,000 IU, vitamin E 10,000 IU/kg.