. 2020 May 5;98(5):skaa116. doi: 10.1093/jas/skaa116

Table 10.

Effect of AFEX wheat straw diet and a recombinant fibrolytic enzyme on acetamide concentrations (mg/kg) in blood and diaphragm of lambs (n = 8 per treatment)

	Control		AFEX¹			P-value
Item	Enz− ²	Enz+ ³	Enz−	Enz+	SEM	Diet	Enz⁴	Diet × Enz
Acetamide in blood
Day 0	0.57^a	0.83^a	0.96^a	0.62^a	1.516	<0.001	0.39	<0.001
Day 7	1.32^b	16.09^a	40.22^a	30.78^a
Day 14	1.09^c	1.58^c	30.83^a	6.16^b
1 d before slaughter	1.01^b	1.32^b	9.50^a	9.87^a
Acetamide in diaphragm	1.83	1.47	8.27	11.88	1.154	<0.001	0.73	0.08

¹AFEX = ammonia fiber expansion treatment (30% AFEX straw pellets on a dietary DM basis replacing alfalfa hay pellets).

²Enz− = diet without enzyme.

³Enz+ = diet with enzyme.

⁴Enz = recombinant fibrolytic enzyme treatment effect (XYL10C, a β-1,4-xylanase, EC 3.2.1.8, GH10 from Aspergillus niger).

^a–cWithin a row, means without a common superscript differ (P < 0.05).